A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells
نویسندگان
چکیده
The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.
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عنوان ژورنال:
- PLoS ONE
دوره 3 شماره
صفحات -
تاریخ انتشار 2008